product summary
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company name :
HUABIO
product type :
antibody
product name :
Osteoprotegerin
catalog :
R1608-4
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/1,000 dilution.
Lane 1: 293 cell lysate
Lane 2: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 55 kDa
Exposure time: 1 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1608-4) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of K-562 cells labeling Osteoprotegerin with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of Neuro-2a cells labeling Osteoprotegerin with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
R1608-4
Target name :
Osteoprotegerin
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Osteoprotegerin aa 1-222 / 401.
Uniprot id :
O00300>SwissProt: O00300 Human;SwissProt: O08712 Mouse;SwissProt: O08727 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
293 cell lysate, K562 cell lysate, K-562, Neuro-2a, C6, human lung cancer tissue, human breast cancer tissue, human kidney tissue, mouse kidney tissue, mouse brain tissue.
Molecular wt :
46 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:100-1:500
;IHC-P: 1:50-1:200
;FC: 1μg/mL
Pic img4 :
https://storage.huabio.cn/huabio/productImg/R1608-4_4.jpg
Pic legend4 :
Immunocytochemistry analysis of C6 cells labeling Osteoprotegerin with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/R1608-4_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/R1608-4_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/R1608-4_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/R1608-4_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/R1608-4_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/R1608-4_10.jpg
Pic legend10 :
Flow cytometric analysis of K-562 cells labeling Osteoprotegerin.
Cells were fixed and permeabilized. Then stained with the primary antibody (R1608-4, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img11 :
https://storage.huabio.cn/huabio/productImg/R1608-4_11.jpg
Pic legend11 :
Flow cytometric analysis of C6 cells labeling Osteoprotegerin.
Cells were fixed and permeabilized. Then stained with the primary antibody (R1608-4, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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