HDAC2 | HUABIO R1601-7 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HDAC2

catalog :

R1601-7

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate Lane 4: PC-12 cell lysate

image 2 :

All lanes: Western blot analysis of HDAC2 with anti-HDAC2 antibody (R1601-7) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: HDAC2 knockout Hela whole cell lysate (10 µg). R1601-7 was shown to specifically react with HDAC2 in wild-type Hela cells. No bands were observed when HDAC2 knockout sample were tested. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1601-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1601-7

Target name :

HDAC2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within C-terminal human Histone Deacetylase 2.

Uniprot id :

Q92769>SwissProt: Q92769 Human;SwissProt: P70288 Mouse;SwissProt: B1WBY8 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SH-SY5Y cell lysate, 293T cell lysate, Hela cell lysate, PC-12 cell lysate, Wild-type Hela whole cell lysate, LOVO, NIH/3T3, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000;IF-Cell: 1:500-1:2,000;IHC-P: 1:100-1:200;FC: 1:50-1:100

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1601-7_4.jpg

Pic legend4 :

ICC staining HDAC2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1601-7_5.jpg

Pic legend5 :

ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1601-7_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1601-7_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1601-7_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/R1601-7_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/R1601-7_10.jpg

Pic legend10 :

Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage