OGG1 | HUABIO R1512-5 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

OGG1

catalog :

R1512-5

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of OGG1 on different lysates with Rabbit anti-OGG1 antibody (R1512-5) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: F9 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-5) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-OGG1 antibody (R1512-5) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-5) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-OGG1 antibody (R1512-5) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

R1512-5

Target name :

OGG1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human OGG1 aa 269-345 / 345.

Uniprot id :

O15527>SwissProt: O15527 Human;SwissProt: O08760 Mouse;SwissProt: O70249 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, F9 cell lysate, mouse skeletal muscle tissue, mouse liver tissue, rat liver tissue, human liver tissue, human liver cancer tissue, human kidney tissue.

Molecular wt :

Predicted band size: 39 kDa

Subcellular location :

Nucleus, nucleoplasm, Nucleus speckle, Nucleus matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1512-5_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-OGG1 antibody (R1512-5) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1512-5_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGG1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1512-5_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-OGG1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1512-5_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGG1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage