CXCR7 | HUABIO R1512-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CXCR7

catalog :

R1512-3

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CXCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: Jurkat cell lysate

image 2 :

ICC staining of CXCR7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-3, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of CXCR7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-3, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

R1512-3

Target name :

CXCR7

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C terminal human CXCR7.

Uniprot id :

P25106>SwissProt: P25106 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, MCF-7 cell lysate, Jurkat cell lysate, Hela, HUVEC, MCF-7.

Molecular wt :

Predicted band size: 41 kDa.

Subcellular location :

Cell membrane, Cytoplasm, Endosome, Membrane

Concentration :

1 mg/ml.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1512-3_4.jpg

Pic legend4 :

Flow cytometric analysis of CXCR7 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-3, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage