product summary
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company name :
HUABIO
product type :
antibody
product name :
Myosin Light Chain 2
catalog :
R1512-19
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Myosin Light Chain 2 on different lysates with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/2,000 dilution.
Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: C2C12 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: Mouse heart tissue lysate (20 µg/Lane)
Lane 5: Mouse skeletal tissue lysate (20 µg/Lane)
Lane 6: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of A431 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of C2C12 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1.000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
R1512-19
Target name :
Myosin Light Chain 2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166.
Uniprot id :
P10916>SwissProt: P10916 Human;SwissProt: P51667 Mouse;SwissProt: P08733 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
A431 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse skeletal tissue lysate, Rat heart tissue lysate, A431, C2C12, C6, mouse heart tissue, rat heart tissue.
Molecular wt :
Predicted band size: 19 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IF-Cell: 1:1,000
;IHC-P: 1:50-1:200
;FC: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/R1512-19_4.jpg
Pic legend4 :
Immunocytochemistry analysis of C6 cells labeling Myosin Light Chain 2 with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myosin Light Chain 2 antibody (R1512-19) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/R1512-19_5.jpg
Pic legend5 :
Flow cytometric analysis of A431 cells labeling Myosin Light Chain 2.
Cells were fixed and permeabilized. Then stained with the primary antibody (R1512-19, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/R1512-19_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/R1512-19_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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