ACO1 | HUABIO R1512-16 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ACO1

catalog :

R1512-16

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (R1512-16) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-16) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining ACO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining ACO1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1512-16

Target name :

ACO1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ACO1 aa 1-273 / 889.

Uniprot id :

P21399>SwissProt: P21399 Human;SwissProt: P28271 Mouse;SwissProt: Q63270 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse liver tissue lysate, rat liver tissue lysate, Hela, HepG2, human thyroid tissue, human kidney tissue, mouse thyroid tissue, mouse ovary tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 98 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1512-16_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ACO1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1512-16_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACO1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1512-16_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ACO1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1512-16_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ACO1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1512-16_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACO1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage