ESD | HUABIO R1511-9 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ESD

catalog :

R1511-9

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ESD on different lysates with Rabbit anti-ESD antibody (R1511-9) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-ESD KD cell lysate (10 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-9) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ESD on K562 cell (1) and mouse intestine tissue (2) lysate using anti-ESD antibody at 1/1,000 dilution.

image 3 :

ICC staining ESD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1511-9

Target name :

ESD

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ESD aa 1-282 / 282.

Uniprot id :

P10768>SwissProt: P10768 Human;SwissProt: Q9R0P3 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K562 cell lysate, mouse intestine tissue lysate, Hela, LOVO, SW480, human prostate tissue, human pancreas tissue, mouse colon tissue.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cytoplasm, Cytoplasmic vesicle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1511-9_4.jpg

Pic legend4 :

ICC staining ESD in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1511-9_5.jpg

Pic legend5 :

ICC staining ESD in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1511-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ESD antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1511-9_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ESD antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1511-9_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-e=ESD antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage