Myosin light chain kinase / MLCK | HUABIO R1511-26 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Myosin light chain kinase / MLCK

catalog :

R1511-26

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody.

image 2 :

Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody.

image 3 :

Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody.

product information

SKU :

R1511-26

Target name :

Myosin light chain kinase / MLCK

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IHC-P,FC,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein with Human MLCK aa 230-470 / 1,914.

Uniprot id :

Q15746>SwissProt: Q15746 Human;SwissProt: Q6PDN3 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF-7, SW480, mouse colon tissue, mouse smooth muscle tissue, human kidney tissue lysates.

Molecular wt :

Predicted band size: 211 kDa

Subcellular location :

Cytoplasm, Cell projection, Cleavage furrow

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:100 ;WB: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1511-26_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1511-26_5.jpg

Pic legend5 :

Flow cytometric analysis of MCF-7 cells with MYLK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1511-26_6.jpg

Pic legend6 :

Western blot analysis of Myosin light chain kinase / MLCK on human kidney tissue lysates with Rabbit anti-Myosin light chain kinase / MLCK antibody (R1511-26) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 211 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage