CLOCK | HUABIO R1511-2 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CLOCK

catalog :

R1511-2

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CLOCK on different lysates with Rabbit anti-CLOCK antibody (R1511-2) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Human brain tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lane 8: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.

image 3 :

Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.

product information

SKU :

R1511-2

Target name :

CLOCK

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse CLOCK 829-855/855.

Uniprot id :

O15516>SwissProt: O15516 Human;SwissProt: O08785 Mouse;SwissProt: Q9WVS9 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat kidney tissue lysate, PC-3M, SW480, Hela, 293, human kidney tissue, mouse testis tissue, mouse skeletal muscle tissue, human colon cancer tissue.

Molecular wt :

Predicted band size: 95 kDa

Subcellular location :

Cytoplasm, Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1511-2_4.jpg

Pic legend4 :

Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1511-2_5.jpg

Pic legend5 :

Immunocytochemical staining of 293 cells using anti-CLOCK rabbit polyclonal antibody.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1511-2_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-CLOCK rabbit polyclonal antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1511-2_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CLOCK rabbit polyclonal antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1511-2_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-CLOCK rabbit polyclonal antibody.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/R1511-2_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-CLOCK rabbit polyclonal antibody.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/R1511-2_10.jpg

Pic legend10 :

Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage