IL-8 | HUABIO R1511-15 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IL-8

catalog :

R1511-15

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (R1511-15) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining IL-8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Flow cytometric analysis of HUVEC cells with IL-8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

product information

SKU :

R1511-15

Target name :

IL-8

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein of Human IL-8 aa 1-99 / 99.

Uniprot id :

P10145>SwissProt: P10145 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate, 293T, HUVEC.

Molecular wt :

Predicted band size: 11 kDa

Subcellular location :

Secreted

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:50 ;FC: 1:100

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage