Calponin-1 | HUABIO R1510-7 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Calponin-1

catalog :

R1510-7

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining of Calponin-1 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1510-7, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Calponin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Calponin-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

R1510-7

Target name :

Calponin-1

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Calponin-1 aa 248-297 / 297.

Uniprot id :

P51911>SwissProt: P51911 Human;SwissProt: Q08091 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

NIH/3T3, mouse smooth muscle tissue, human fetal skeletal muscle tissue, Hela.

Molecular wt :

33 kDa

Subcellular location :

Cytoskeleton, focal adhesion.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:100 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1510-7_4.jpg

Pic legend4 :

Flow cytometric analysis of Calponin-1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-7, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage