ABCA1 | HUABIO R1510-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ABCA1

catalog :

R1510-42

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ABCA1 on different lysates with Rabbit anti-ABCA1 antibody (R1510-42) at 1/500 dilution. Lane 1: Rat brain tissue lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 254 kDa Observed band size: 254 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemical staining of PC-3M cells using anti-ABCA1 rabbit polyclonal antibody.

image 3 :

Immunocytochemistry analysis of HepG2 cells labeling ABCA1 with Rabbit anti-ABCA1 antibody (R1510-42) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ABCA1 antibody (R1510-42) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

R1510-42

Target name :

ABCA1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ABCA1 aa 21-70 / 2,261.

Uniprot id :

O95477>SwissProt: O95477 Human;SwissProt: P41233 Mouse;SwissProt: Q80ZB2 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat brain tissue lysate, Jurkat cell lysate, mouse brain tissue lysate, PC-3M, human liver tissue, human pancreas tissue, mouse prostate tissue, mouse pancreas tissue, Jurkat.

Molecular wt :

Predicted band size: 254 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1510-42_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-ABCA1 rabbit polyclonal antibody

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1510-42_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-ABCA1 rabbit polyclonal antibody.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1510-42_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-ABCA1 rabbit polyclonal antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1510-42_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-ABCA1 rabbit polyclonal antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1510-42_8.jpg

Pic legend8 :

Flow cytometric analysis of Jurkat cells with ABCA1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage