PHD2 | HUABIO R1510-40 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PHD2

catalog :

R1510-40

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PHD2 on different lysates with Rabbit anti-PHD2 antibody (R1510-40) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PHD2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis on mouse brain lysates using anti-PHD2 rabbit polyclonal antibody.

image 3 :

Immunocytochemical staining of PC-12 cells using anti-PHD2 rabbit polyclonal antibody.

product information

SKU :

R1510-40

Target name :

PHD2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PHD2 aa 377-426 / 426.

Uniprot id :

Q9GZT9>SwissProt: Q9GZT9 Human;SwissProt: Q91YE3 Mouse;SwissProt: P59722 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

PC-12, human kidney tissue, mouse kidney tissue, human pancreas tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 46kDa

Subcellular location :

Cytoplasm, Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1510-40_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-PHD2 rabbit polyclonal antibody.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1510-40_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-PHD2 rabbit polyclonal antibody.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1510-40_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-PHD2 rabbit polyclonal antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1510-40_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-PHD2 rabbit polyclonal antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1510-40_8.jpg

Pic legend8 :

Flow cytometric analysis of SH-SY-5Y cells with PHD2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage