Peroxiredoxin 2 | HUABIO R1510-37 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Peroxiredoxin 2

catalog :

R1510-37

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Mouse heart tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (R1510-37) at 1/5,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si Peroxiredoxin 2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-37) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

R1510-37

Target name :

Peroxiredoxin 2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PRDX2 aa 149-198 / 198.

Uniprot id :

P32119>SwissProt: P32119 Human;SwissProt: Q61171 Mouse;SwissProt: P35704 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, PC-12 cell lysate, A549 cell lysate, 293T cell lysate, mouse heart tissue lysate, Hela, SHG-44, SH-SY5Y, human liver tissue, human kidney tissue, mouse brain tissue, mouse prostate tissue, mouse heart tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:100-1:500

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1510-37_4.jpg

Pic legend4 :

ICC staining Peroxiredoxin 2/PRDX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1510-37_5.jpg

Pic legend5 :

ICC staining Peroxiredoxin 2/PRDX2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1510-37_6.jpg

Pic legend6 :

ICC staining Peroxiredoxin 2/PRDX2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1510-37_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1510-37_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/R1510-37_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/R1510-37_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/R1510-37_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Peroxiredoxin 2/PRDX2 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage