nNOS | HUABIO R1510-28 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

nNOS

catalog :

R1510-28

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/5,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (R1510-28, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (R1510-28) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 161 kDa Observed band size: 161 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-28) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

R1510-28

Target name :

nNOS

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human nNOS.

Uniprot id :

P29475>SwissProt: P29475 Human;SwissProt: Q9Z0J4 Mouse;SwissProt: P29476 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse brain tissue lysate, Rat brain tissue lysate, mouse colon tissue, rat colon tissue, SHG-44, SK-BR-3, HepG2, N2A.

Molecular wt :

Predicted band size: 161 kDa

Subcellular location :

Cell membrane, Cell projection

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:10,000-1:50,000 ;FC: 1:50-1:100 ;IHC-Fr: 1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1510-28_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-nNOS antibody (R1510-28) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1510-28) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1510-28_5.jpg

Pic legend5 :

Western blot analysis on SHG-44(1) and mouse brain(2) lysates using anti-nNOS rabbit polyclonal antibody.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1510-28_6.jpg

Pic legend6 :

Immunocytochemical staining of SK-BR-3 cells using anti-nNOS rabbit polyclonal antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/R1510-28_7.jpg

Pic legend7 :

Immunocytochemical staining of HepG2 cells using anti-nNOS rabbit polyclonal antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/R1510-28_8.jpg

Pic legend8 :

Flow cytometric analysis of N2A cells with nNOS antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage