All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody (R1508-1) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Lamin B1 knockdown Hela whole cell lysate (10 µg). R1508-1 was shown to specifically react with Lamin B1 in wild-type Hela cells. Weakened band was observed when Lamin B1 knockdown sample was tested. Wild-type and Lamin B1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1508-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.