ERp72 | HUABIO R1409-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERp72

catalog :

R1409-1

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERp72 on different lysates with Rabbit anti-ERp72 antibody (R1409-1) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: PANC-1 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Human liver tissue lysate (40 µg/Lane) Lane 7: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 73 kDa Observed band size: 73 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1409-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining ERp72 in A549 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining ERp72 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1409-1) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

R1409-1

Target name :

ERp72

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminalhuman ERP72.

Uniprot id :

P13667>SwissProt: P13667 Human;SwissProt: P08003 Mouse;SwissProt: P38659 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, PANC-1 cell lysate, PC-12 cell lysate, human liver tissue lysate, mouse liver tissue lysate, A549, PC-12, human lung cancer tissue.

Molecular wt :

Predicted band size: 73 kDa

Subcellular location :

Endoplasmic reticulum lumen, Melanosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:5,000 ;IF-Cell:1:500 ;IHC-P:1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1409-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1409-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage