ALDH2 | HUABIO R1407-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH2

catalog :

R1407-3

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (R1407-3) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SK-Br-3 cell lysate (15 µg/Lane) Lane 4: THP-1 cell lysate (15 µg/Lane) Lane 5: Human liver tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

image 3 :

ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

product information

SKU :

R1407-3

Target name :

ALDH2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human ALDH2 aa 18-80.

Uniprot id :

P05091>SwissProt: P05091 Human;SwissProt: P47738 Mouse;SwissProt: P11884 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat lung tissue lysate, Hela, HepG2.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200

more info or order :

HUABIO product webpage