Glucose Transporter GLUT4 | HUABIO R1402-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Glucose Transporter GLUT4

catalog :

R1402-3

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Glucose Transporter GLUT4 on different lysates with Rabbit anti-Glucose Transporter GLUT4 antibody (R1402-3) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate (no heat) Lane 2: Mouse skeletal muscle tissue lysate (no heat) Lane 3: Rat skeletal muscle tissue lysate (no heat) Lane 4: Rat kidney tissue lysate (low expression) (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining GLUT4 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-GLUT4 antibody. Counter stained with hematoxylin.

product information

SKU :

R1402-3

Target name :

Glucose Transporter GLUT4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Glucose Transporter GLUT4 aa 460-509 / 509.

Uniprot id :

P14672>SwissProt: P14672 Human;SwissProt: P14142 Mouse;SwissProt: P19357 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, mouse heart tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cell membrane, Endomembrane system, Cytoplasm, perinuclear region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage