NSE | HUABIO R1401-6 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NSE

catalog :

R1401-6

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, zebrafish

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (R1401-6) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSE KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1401-6) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis on different cell lysates using anti-NSE rabbit polyclonal antibodies.

image 3 :

Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysate using anti-NSE antibody at 1/500 dilution.

product information

SKU :

R1401-6

Target name :

NSE

Species reactivity :

Human,Mouse,Rat,Zebrafish,Hybrid fish (crucian-carp)

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human NSE.

Uniprot id :

P09104>SwissProt: P09104 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela, NIH/3T3, F9, human brain, mouse brain, A172, SHG-44, N2A hybrid fish (crucian-carp) brain tissue lysate.

Molecular wt :

47 kDa

Subcellular location :

Cytoplasm, cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2000-1:5,000 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1401-6_4.jpg

Pic legend4 :

ICC staining NSE in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage