product summary
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company name :
HUABIO
product type :
antibody
product name :
Y14
catalog :
R1401-10
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Y14 on SH-SY5Y cell lysates with Rabbit anti-Y14 antibody (R1401-10) at 1/500 dilution.
image 2 :
ICC staining Y14 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
image 3 :
Immunocytochemistry analysis of MCF-7 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
product information
SKU :
R1401-10
Target name :
Y14
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Y14 aa 125-174 / 174.
Uniprot id :
Q9Y5S9>SwissProt: Q9Y5S9 Human;SwissProt: Q9CWZ3 Mouse;SwissProt: Q27W01 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
SH-SY5Y, MCF-7, RWPE-1, SW620, mouse kidney tissue, mouse heart tissue.
Molecular wt :
Predicted band size: 20kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
;IHC-P: 1:200
;IF-Cell: 1:50-1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/R1401-10_4.png
Pic legend4 :
Immunocytochemistry analysis of RWPE-1 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/R1401-10_5.png
Pic legend5 :
Immunocytochemistry analysis of SW620 cells labeling Y14 with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Y14 antibody (R1401-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/R1401-10_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Y14 rabbit polyclonal antibody.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/R1401-10_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Y14 rabbit polyclonal antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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