DPY30 | HUABIO R1312-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DPY30

catalog :

R1312-4

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of NCCIT cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of F9 cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

product information

SKU :

R1312-4

Target name :

DPY30

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Full length protein.

Uniprot id :

Q9C005>SwissProt: Q9C005 Human;SwissProt: Q99LT0 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

NCCIT , F9, D3, human lung carcinoma tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 11kDa

Subcellular location :

Nucleus. Golgi apparatus

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:100-1:200 ;IF-Cell: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1312-4_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- DPY30 rabbit polyclonal antibody.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1312-4_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPY30 rabbit polyclonal antibody.

more info or order :

HUABIO product webpage