BMPR1B | HUABIO R1311-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BMPR1B

catalog :

R1311-4

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis on SHG-44 cell lysates using anti- BMPR1B rabbit polyclonal antibodies.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling BMPR1B with Rabbit anti-BMPR1B antibody (R1311-4) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BMPR1B antibody (R1311-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using anti- BMPR1B rabbit polyclonal antibody.

product information

SKU :

R1311-4

Target name :

BMPR1B

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human BMPR1B aa 14-126.

Uniprot id :

O00238>SwissProt: O00238 Human;SwissProt: P36898 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HepG2, SHG-44, human ovarian carcinoma tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 57kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1000;IHC-P: 1:100-1:500;IF-Cell: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1311-4_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-BMPR1B antibody (R1311-4) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1311-4) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage