Cdk2 | HUABIO R1309-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cdk2

catalog :

R1309-3

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cdk2 on different cell lysates using anti-Cdk2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: F9 cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse thymus tissue lysate Lane 5: Human liver tissue lysate

image 2 :

Western blot analysis of Cdk2 on different lysates with Rabbit anti-Cdk2 antibody (R1309-3) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Cdk2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. R1309-3 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1309-3, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining Cdk2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

R1309-3

Target name :

Cdk2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Cdk2 aa 249-298 / 298.

Uniprot id :

P24941>SwissProt: P24941 Human;SwissProt: P97377 Mouse;SwissProt: Q63699 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

A549 cell lysate, F9 cell lysate, PC-12 cell lysate, Mouse thymus tissue lysate, Human liver tissue lysate, A549, human tonsil tissue, human breast cancer tissue, Hela.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, Nucleus, Cytoskeleton, Endosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1309-3_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/R1309-3_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/R1309-3_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells with Cdk2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage