HMGB1 | HUABIO R1309-12 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HMGB1

catalog :

R1309-12

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (R1309-12) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockout Raw264.7 whole cell lysate. R1309-12 was shown to specifically react with HMGB1 in wild-type Raw264.7 cells. No band was observed when HMGB1 knockout sample was tested. Wild-type and HMGB1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (R1309-12, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 2 :

Western blot analysis on MCF-7 (1), PC12 (2) and F9 (3) cell lysates using anti-HMGB1 rabbit polyclonal antibodies.

image 3 :

Immunofluorescent staining of Hela cells using anti- HMGB1 rabbit polyclonal antibody.

product information

SKU :

R1309-12

Target name :

HMGB1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human HMGB1.

Uniprot id :

P09429>SwissProt: P09429 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Wild-type Raw264.7 whole cell lysate, MCF-7 cell lysate, PC12 cell lysate, F9 cell lysate, Hela, mouse spleen tissue.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Nucleus, Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000;IF-Cell: 1:200-1:500;IHC-P: 1:200;FC: 1:100

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1309-12_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- HMGB1 rabbit polyclonal antibody.

more info or order :

HUABIO product webpage