product summary
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company name :
HUABIO
product type :
antibody
product name :
GFAP
catalog :
R1308-9
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis on human brain tissue lysates using anti-GFAP rabbit polyclonal antibodies.
image 2 :
Western blot analysis of GFAP on mouse brain tissue lysates with Rabbit anti-GFAP antibody (R1308-9) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunofluorescent staining of A172 cells using anti- GFAP rabbit polyclonal antibody.
product information
SKU :
R1308-9
Target name :
GFAP
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N-terminal human GFAP.
Uniprot id :
P14136>SwissProt: P14136 Human;SwissProt: P03995 Mouse;SwissProt: P47819 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Human brain tissue lysates, mouse brain tissue lysates, A172, human brain tissue, rat hippocampus tissue.
Molecular wt :
Predicted band size: 50 kDa
Subcellular location :
Cytoplasm, Intermediate filament.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:50-1:200
;IHC-P: 1:200
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/R1308-9_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- GFAP rabbit polyclonal antibody.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/R1308-9_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GFAP antibody (R1308-9) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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