product summary
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company name :
HUABIO
product type :
antibody
product name :
p53
catalog :
R1308-7
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO R1308-7 image 1
Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (R1308-7) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-468 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO R1308-7 image 2
Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (R1308-7) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-p53 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-7) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
HUABIO R1308-7 image 3
Immunofluorescent staining of A431 cells using anti- p53 rabbit polyclonal antibody.
product information
SKU :
R1308-7
Target name :
p53
Species reactivity :
Human
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within C-terminal human P53.
Uniprot id :
P04637>SwissProt: P04637 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
MDA-MB-231 cell lysate, HEK-293 cell lysate, MDA-MB-468 cell lysate, A431.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Cytoplasm, Nucleus, Cytoskeleton, Endoplasmic reticulum, Mitochondrion.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IF-Cell: 1:100
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!