Vimentin | HUABIO R1308-6 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Vimentin

catalog :

R1308-6

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (R1308-6) at 1/2,000 dilution. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 56 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-6) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

All lanes: Western blot analysis of Vimentin with anti-Vimentin antibody (R1308-6) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Vimentin knockout Hela whole cell lysate (10 µg). R1308-6 was shown to specifically react with Vimentin in wild-type Hela cells. No band was observed when Vimentin knockout sample was tested. Wild-type and Vimentin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1308-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunofluorescent staining of Hela cells using anti- vimentin rabbit polyclonal antibody.

product information

SKU :

R1308-6

Target name :

Vimentin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within residues of Human Vimentin aa 417-466 / 466.

Uniprot id :

P08670>SwissProt: P08670 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, A549 cell lysate, 293T cell lysate, Hela, human breast carcinoma tissue, Wild-type Hela whole cell lysate.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000;IF-Cell: 1:100-1:200;IHC-P: 1:200

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1308-6_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-vimentin rabbit polyclonal antibody.

more info or order :

HUABIO product webpage