Active+Pro Caspase-9 | HUABIO R1308-12 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Active+Pro Caspase-9

catalog :

R1308-12

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (R1308-12) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate (20 µg/Lane) Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 46/37/35 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (R1308-12) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si Caspase-9 cell lysate (10 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. R1308-12 was shown to specifically react with Active+Pro Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Active+Pro Caspase-9 sample was tested. Hela-si NT and Hela-si Active+Pro Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1308-12, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caspase-9 rabbit polyclonal antibody.

product information

SKU :

R1308-12

Target name :

Active+Pro Caspase-9

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Caspase-9 aa 299-313/416.

Uniprot id :

P55211>SwissProt: P55211 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, HeLa treated with 3μM staurosporine for 4 hours cell lysate, human lung carcinoma tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000- 1:2,000 ;IHC-P: 1:200

Advanced Validation :

Cell treatment (CT),Knockdown (KD)

more info or order :

HUABIO product webpage