KLF4 | HUABIO R1308-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KLF4

catalog :

R1308-1

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KLF4 on different lysates with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,000 dilution. Lane 1: F9 cell lysate (20 µg/Lane) Lane 2: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 27 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1308-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis on 293 (1) and F9 (2) cell lysates using anti-KLF4 rabbit polyclonal antibodies.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-1) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

R1308-1

Target name :

KLF4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human KLF4 aa 406-458.

Uniprot id :

O43474>SwissProt: O43474 Human;SwissProt: Q60793 Mouse;Entrez Gene: 114505 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

F9 cell lysate, Rat liver tissue lysate, 293 cell lysates, human colon tissue, human skin tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P:1:200-1:1,500 ;FC: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/R1308-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-KLF4 antibody (R1308-1) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1308-1) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage