Nucleolin | HUABIO R1307-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Nucleolin

catalog :

R1307-3

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (R1307-3) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lane 5: A431 cell lysate Lane 6: HepG2 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunofluorescent staining of Hela cells using anti- nucleolin rabbit polyclonal antibody.

image 3 :

Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-nucleolin rabbit polyclonal antibody.

product information

SKU :

R1307-3

Target name :

Nucleolin

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Nucleolin aa 271-320 / 710.

Uniprot id :

P19338>SwissProt: P19338 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K-562 cell lysate, HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, Hela, human ovary tissue.

Molecular wt :

Predicted band size: 77 kDa

Subcellular location :

Cell nucleus, cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:200

more info or order :

HUABIO product webpage