product summary
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company name :
HUABIO
product type :
antibody
product name :
Nucleolin
catalog :
R1307-2
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO R1307-2 image 1
Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (R1307-2) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: A431 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-2) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO R1307-2 image 2
ICC staining nucleolin in mouse embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
product information
SKU :
R1307-2
Target name :
Nucleolin
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Nucleolin aa 201-250 / 710.
Uniprot id :
P19338>SwissProt: P19338 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, mouse embryonic stem.
Molecular wt :
Predicted band size: 77 kDa
Subcellular location :
Nucleus, nucleolus, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IF-Cell: 1:100-1:200
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!