product summary
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company name :
HUABIO
product type :
antibody
product name :
6X His tag
catalog :
R1207-2
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of 6X His tag on C-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1207-2, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with R1207-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M0812-3 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
Lane 1: His Tag fusion protein lysate (input).
Lane 2: 0812-1 IP in His Tag fusion protein lysate.
Lane 3: R1207-2 IP in His Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of R1207-2 in His Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
product information
SKU :
R1207-2
Target name :
6X His tag
Species reactivity :
Species independent
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide immune sequence is HHHHHHHHC.
Uniprot id :
>
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
C-terminal His-tagged recombinant protein lysates.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IP: 2-5 µg/ml.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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