Cytochrome C | HUABIO M1701-9 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytochrome C

catalog :

M1701-9

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10-E11-G2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cytochrome C on different lysates with Mouse anti-Cytochrome C antibody (M1701-9) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining Cytochrome C (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Cytochrome C (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

M1701-9

Target name :

Cytochrome C

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cytochrome C aa 2-60.

Uniprot id :

P99999>SwissProt: P99999 Human;SwissProt: P62897 Mouse;SwissProt: P62898 Rat

Host :

Mouse

Clone number :

10-E11-G2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, Hela, HepG2, MCF-7, human liver tissue, human spleen tissue, human kidney tissue, mouse heart tissue.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Mitochondrion

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:600

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1701-9_4.jpg

Pic legend4 :

ICC staining Cytochrome C (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1701-9_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1701-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1701-9_7.png

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Cytochrome C antibody (M1701-9) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-9) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1701-9_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage