PTBP1 | HUABIO M1701-7 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PTBP1

catalog :

M1701-7

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5C1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (M1701-7) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: SW620 cell lysate (20 µg/Lane) Lane 7: A431 cell lysate (20 µg/Lane) Lane 8: A549 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: C6 cell lysate (20 µg/Lane) Lane 11: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PTBP1 on mouse kidney tissue lysates with Mouse anti-PTBP1 antibody (M1701-7) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-7) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling PTBP1 with Mouse anti-PTBP1 antibody (M1701-7) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PTBP1 antibody (M1701-7) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M1701-7

Target name :

PTBP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PTBP1 aa 1-50 / 531.

Uniprot id :

P26599>SwissProt: P26599 Human;SwissProt: P17225 Mouse;SwissProt: Q00438 Rat

Host :

Mouse

Clone number :

5C1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, K-562 cell lysate, 293T cell lysate, SW620 cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human kidney tissue lysate, mouse kidney tissue lysates, HeLa, human kidney tissue, human colon cancer tissue, human breast cancer tissue, human placental tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:400-1:2,000 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1701-7_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1701-7_5.png

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1701-7_6.png

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1701-7_7.png

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human placental tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1701-7_8.png

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/M1701-7_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-PTBP1 antibody (M1701-7) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-7) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage