gamma tubulin | HUABIO M1701-13 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

gamma tubulin

catalog :

M1701-13

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10-A4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of gamma tubulin on different lysates using anti-gamma tubulin antibody at 1/2,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: Human fetal brain tissue

image 2 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

product information

SKU :

M1701-13

Target name :

gamma tubulin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human gamma tubulin aa 402-451 / 451.

Uniprot id :

P23258>SwissProt: P23258 Human;SwissProt: P83887 Mouse;SwissProt: P83888 Rat

Host :

Mouse

Clone number :

10-A4

Isotype :

IgG2a

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Rat epididymis tissue, human tonsil tissue, human colon cancer tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cytoplasm, cytoskeleton, centrosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1701-13_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1701-13_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1701-13_6.jpg

Pic legend6 :

Immunocytochemistry analysis of Hela cells labeling gamma tubulin with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at RT, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage