PP2A alpha + beta | HUABIO M1603-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PP2A alpha + beta

catalog :

M1603-4

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A0-G7

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PP2A alpha + beta on different lysates with Mouse anti-PP2A alpha + beta antibody (M1603-4) at 1/2,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: 293T cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: F9 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1603-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

M1603-4

Target name :

PP2A alpha + beta

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PP2A alpha + beta aa 260-309 / 309.

Uniprot id :

P67775>SwissProt: P67775 Human;SwissProt: P62714 Human;SwissProt: P63$330 Mouse;SwissProt: P62715 Mouse;SwissProt: P63331 Rat;SwissProt: P62716 Rat

Host :

Mouse

Clone number :

A0-G7

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela, MCF-7, SHG-44.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, Nucleus, Chromosome, centromere, cytoskeleton, spindle pole.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:100-1:500 ;FC: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1603-4_4.jpg

Pic legend4 :

ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1603-4_5.jpg

Pic legend5 :

Flow cytometric analysis of Hela cells with PP2A(alpha+beta) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage