Haptoglobin | HUABIO M1510-36 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Haptoglobin

catalog :

M1510-36

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10-F5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Haptoglobin on human plasma lysates with Mouse anti-Haptoglobin antibody (M1510-36) at 1/4,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-36) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1510-36

Target name :

Haptoglobin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,ELISA

Conjugate :

Non-conjugated

Immunogen :

Native protein.

Uniprot id :

P00738>SwissProt: P00738 Human;SwissProt: Q61646 Mouse;SwissProt: P06866 Rat

Host :

Mouse

Clone number :

A10-F5

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human plasma lysates, human liver tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:4,000 ;IHC-P: 1:1,000 ;ELISA: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1510-36_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage