EFTUD2 | HUABIO M1510-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EFTUD2

catalog :

M1510-3

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1-D10-A1

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EFTUD2 on different lysates with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: U-87 MG cell lysate Lane 5: LNCaP cell lysate Lane 6: HepG2 cell lysate Lane 7: K-562 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: Mouse testis tissue lysate Lane 13: Mouse kidney tissue lysate Lane 14: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 109 kDa Observed band size: 120 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1510-3

Target name :

EFTUD2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse Eftud2 aa 1-50 / 972.

Uniprot id :

Q15029>SwissProt: Q15029 Human;SwissProt: O08810 Mouse;Entrez Gene: 287739 Rat

Host :

Mouse

Clone number :

1-D10-A1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, U-87 MG cell lysate, LNCaP cell lysate, HepG2 cell lysate, K-562 cell lysate, C6 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, HeLa, human breast cancer tissue, human colon cancer tissue, human colon tissue, human ovary cancer tissue, mouse kidney tissue, mouse testis tissue, rat testis tissue, NIH/3T3.

Molecular wt :

Predicted band size: 109 kDa

Subcellular location :

Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:500 ;IHC-P: 1:2,000 ;IF-Tissue: 1:500 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1510-3_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1510-3_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1510-3_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1510-3_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1510-3_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-EFTUD2 antibody (M1510-3) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-3) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/M1510-3_9.jpg

Pic legend9 :

Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling EFTUD2 with Mouse anti-EFTUD2 antibody (M1510-3) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1510-3, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/M1510-3_10.jpg

Pic legend10 :

Immunofluorescence analysis of paraffin-embedded rat testis tissue labeling EFTUD2 with Mouse anti-EFTUD2 antibody (M1510-3) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1510-3, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/M1510-3_11.jpg

Pic legend11 :

Immunocytochemistry analysis of HeLa cells labeling EFTUD2 with Mouse anti-EFTUD2 antibody (M1510-3) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EFTUD2 antibody (M1510-3) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/M1510-3_12.jpg

Pic legend12 :

Flow cytometric analysis of HeLa cells labeling EFTUD2. Cells were fixed and permeabilized. Then stained with the primary antibody (M1510-3, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/M1510-3_13.jpg

Pic legend13 :

Flow cytometric analysis of NIH/3T3 cells labeling EFTUD2. Cells were fixed and permeabilized. Then stained with the primary antibody (M1510-3, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage