Fibrinogen | HUABIO M1510-11 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Fibrinogen

catalog :

M1510-11

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D5-B3

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Fibrinogen on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-11, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

product information

SKU :

M1510-11

Target name :

Fibrinogen

Species reactivity :

Human

Applications :

IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

native protein.

Uniprot id :

P02671>SwissProt: P02671 Human;SwissProt: P02675 Human;SwissProt: P02679 Human

Host :

Mouse

Clone number :

D5-B3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysates, human liver tissue, human placenta tissue.

Molecular wt :

Predicted band size: 95/56/52 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P:1:50-1:200 ;WB: 1:500-1:2000

more info or order :

HUABIO product webpage