ALDH2 | HUABIO M1509-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH2

catalog :

M1509-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

E4-D10

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (M1509-1) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1509-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SK-Br-3 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M1509-1

Target name :

ALDH2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ALDH2 aa 468-517 / 517.

Uniprot id :

P05091>SwissProt: P05091 Human;SwissProt: P47738 Mouse;SwissProt: P11884 Rat

Host :

Mouse

Clone number :

E4-D10

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, SK-Br-3, HeLa, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human gastric cancer tissue, mouse colon tissue, mouse stomach tissue.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1509-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-ALDH2 Mouse mAb.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1509-1_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-ALDH2 Mouse mAb.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1509-1_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-ALDH2 Mouse mAb.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1509-1_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin- embedded human gastric cancer tissue using anti-ALDH2 Mouse mAb.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1509-1_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-ALDH2 Mouse mAb.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/M1509-1_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin- embedded mouse stomach tissue using anti-ALDH2 Mouse mAb.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/M1509-1_10.jpg

Pic legend10 :

Flow cytometric analysis of HepG2 cells with ALDH2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage