Cyclin B1 | HUABIO M1508-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cyclin B1

catalog :

M1508-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C2-F2

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cyclin B1 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 58 kDa

image 2 :

ICC staining Cyclin B1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

image 3 :

ICC staining Cyclin B1 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

product information

SKU :

M1508-1

Target name :

Cyclin B1

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide (KLH-coupled) within human Cyclin B1 aa 391-433.

Uniprot id :

P14635>SwissProt: P14635 Human

Host :

Mouse

Clone number :

C2-F2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K562 cell lysates, HepG2, LOVO.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Cytoplasm, Nucleus, cytoskeleton, microtubule organizing center, centrosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB:1:500 ;IF-Cell:1:200-1:500

more info or order :

HUABIO product webpage