SIRT1 | HUABIO M1506-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SIRT1

catalog :

M1506-3

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7-C5-B2

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SIRT1 on different lysates with Mouse anti-SIRT1 antibody (M1506-3) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 120 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-3) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling SIRT1 with Mouse anti-SIRT1 antibody (M1506-3) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-SIRT1 antibody (M1506-3) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- SIRT1 mouse mAb.

product information

SKU :

M1506-3

Target name :

SIRT1

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human SIRT1 aa 698-747 / 747.

Uniprot id :

Q96EB6>SwissProt: Q96EB6 Human

Host :

Mouse

Clone number :

7-C5-B2

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HAP1 cell lysate, HeLa, human lung carcinoma tissue, human colon carcinoma tissue

Molecular wt :

Predicted band size: 82 kDa

Subcellular location :

Nucleus, PML body, Cytoplasm.

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P: 1:200 ;IF-Cell: 1:100 ;WB: 1:2,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1506-3_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti- SIRT1 mouse mAb.

more info or order :

HUABIO product webpage