Cathepsin B | HUABIO M1506-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cathepsin B

catalog :

M1506-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J11-A11

reactivity :

human, mouse, rat, zebrafish

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cathepsin B on different lysates with Mouse anti-Cathepsin B antibody (M1506-1) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SW480 cells labeling Cathepsin B with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of PC-12 cells labeling Cathepsin B with Mouse anti-Cathepsin B antibody (M1506-1) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cathepsin B antibody (M1506-1) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M1506-1

Target name :

Cathepsin B

Species reactivity :

Human,Zebrafish,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cathepsin B aa 300-338/338.

Uniprot id :

P07858>SwissProt: P07858 Human;SwissProt: P10605 Mouse;SwissProt: P00787 Rat

Host :

Mouse

Clone number :

J11-A11

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SW480 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, SW480, PC-12.

Molecular wt :

Predicted band size: 38 kDa

Subcellular location :

Lysosome, Melanosome, Secreted, Apical cell membrane.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:500

more info or order :

HUABIO product webpage