JAK2 | HUABIO M1501-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

JAK2

catalog :

M1501-8

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6-D3

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of JAK2 on different lysates with Mouse anti-JAK2 antibody (M1501-8) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si JAK2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1501-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of JAK2 on different lysates with Mouse anti-JAK2 antibody (M1501-8) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: TF-1 cell lysate Lane 3: Jurkat cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1501-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of K-562 cells labeling JAK2 with Mouse anti-JAK2 antibody (M1501-8) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-JAK2 antibody (M1501-8) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

M1501-8

Target name :

JAK2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human JAK2 aa 883-1,132 / 1,132.

Uniprot id :

O60674>SwissProt: O60674 Human

Host :

Mouse

Clone number :

6-D3

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549 cell lysate, K-562 cell lysate, TF-1 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, C6 cell lysate, K-562, RAW264.7, human kidney tissue, human lung carcinoma tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 130 kDa

Subcellular location :

Cytopasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1501-8_4.jpg

Pic legend4 :

Immunocytochemistry analysis of RAW264.7 cells labeling JAK2 with Mouse anti-JAK2 antibody (M1501-8) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-JAK2 antibody (M1501-8) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1501-8_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-JAK2 antibody (M1501-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1501-8_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-JAK2 antibody (M1501-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/M1501-8_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-JAK2 antibody (M1501-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/M1501-8_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-JAK2 antibody (M1501-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage