Catalase | HUABIO M1501-6 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Catalase

catalog :

M1501-6

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4-G10

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis on mouse liver lysates using anti-catalse mouse mAb.

image 2 :

ICC staining catalse in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Catalase antibody (M1501-6) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-6) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1501-6

Target name :

Catalase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Catalase aa 475-527/527.

Uniprot id :

P04040>SwissProt: P04040 Human;SwissProt: P24270 Mouse;SwissProt: P04762 Rat

Host :

Mouse

Clone number :

4-G10

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Mouse liver lysates, human liver tissue, human liver cancer tissue, human kidney tissue, HepG2.

Molecular wt :

Predicted band size: 60kDa

Subcellular location :

Peroxisome

Concentration :

2 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;WB: 1:2,000-1:5,000 ;IHC-P: 1:100-1:400 ;FC: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1501-6_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Catalase antibody (M1501-6) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-6) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1501-6_5.png

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Catalase antibody (M1501-6) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1501-6) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/M1501-6_6.png

Pic legend6 :

Flow cytometric analysis of HepG2 cells labeling Catalase. Cells were fixed and permeabilized. Then stained with the primary antibody (M1501-6, 1ug/ml) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage