STAT1 | HUABIO M1407-1 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

STAT1

catalog :

M1407-1

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G3-B11

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: MCF7 cell lysate Lane 8: HT-29 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1407-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HT-29 cells labeling STAT1 with Mouse anti-STAT1 antibody (M1407-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-STAT1 antibody (M1407-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1407-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1407-1

Target name :

STAT1

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human STAT1 aa 71-270 / 750.

Uniprot id :

P42224>SwissProt: P42224 Human

Host :

Mouse

Clone number :

G3-B11

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Jurkat cell lysate, A431 cell lysate, HeLa cell lysate, A549 cell lysate, SK-Br-3 cell lysate, SK-MEL-28 cell lysate, MCF7 cell lysate, HT-29 cell lysate, HT-29, human breast cancer tissue, human spleen tissue.

Molecular wt :

Predicted band size: 87/83 kDa

Subcellular location :

Cytoplasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/M1407-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1407-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/M1407-1_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling STAT1 with Mouse anti-STAT1 antibody (M1407-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1407-1, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage