Beta Catenin | HUABIO M1405-6 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Beta Catenin

catalog :

M1405-6

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10-C0-B7

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis on 293T cell lysates using anti- Beta-catenin mouse mAb.

image 2 :

ICC staining Beta-catenin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-catenin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1405-6, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1405-6

Target name :

Beta Catenin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human Beta-catenin.

Uniprot id :

P35222>SwissProt: P35222 Human;SwissProt: Q02248 Mouse;SwissProt: Q9WU82 Rat

Host :

Mouse

Clone number :

10-C0-B7

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela, 293T, human colon carcinoma tissue.

Molecular wt :

85 kDa

Subcellular location :

Cytoplasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100

more info or order :

HUABIO product webpage