E-Cadherin | HUABIO M1405-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

E-Cadherin

catalog :

M1405-3

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3-F9

reactivity :

human, mouse

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (M1405-3) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1405-3) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-E-Cadherin antibody (M1405-3) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1405-3) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

M1405-3

Target name :

E-Cadherin

Species reactivity :

Human,Mouse

Applications :

IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse E-Cadherin aa 350-550.

Uniprot id :

P12830>SwissProt: P12830 Human;SwissProt: P09803 Mouse

Host :

Mouse

Clone number :

3-F9

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human liver tissue, mouse liver tissue.

Molecular wt :

~130kDa

Subcellular location :

Cell membrane

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P: 1:200

more info or order :

HUABIO product webpage