Cdk2 | HUABIO M1403-3 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cdk2

catalog :

M1403-3

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10-D7

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis on different cell lysates using anti- Cdk2 mouse mAb.

image 2 :

Western blot analysis of Cdk2 on different lysates with Mouse anti-Cdk2 antibody (M1403-3) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Cdk2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. M1403-3 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (M1403-3, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-mouse IgG-HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining Cdk2 in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

product information

SKU :

M1403-3

Target name :

Cdk2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Cdk2 aa 249-298 / 298.

Uniprot id :

P24941>SwissProt: P24941 Human

Host :

Mouse

Clone number :

10-D7

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A549, F9, PC12, NCCIT

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, nucleus

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2000 ;IF-Cell: 1:500

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage